cytidine triphosphate synthesis

Glazer RI, Knode MC, Lim MI, Marquez VE. Cytidine 5′-triphosphate (CTP) is a pyrimidine nucleoside triphosphate that is analogous to the better-known ATP, but with cytidine as the base instead of adenine. CTP acts as an inhibitor of the enzyme aspartate carbamoyltransferase, which is used in pyrimidine biosynthesis. These variations probably reflect the species differences in cytidine deaminase, the enzyme that converts cytidine to uridine in the body. A subsidiary of Cambridge Isotope Laboratories, Inc. Informations; About Us; Contact Us; Distributors; Custom Synthesis… The enzyme Cytidine 5’-triphosphate (CTP) synthetase (EC 6.3.4.2) catalyzes a critical reaction in pyrimidine nucleotide biosynthesis as well as phospholipid formation [1-3]. The mechanism of action of the cyclopentenyl analogue of cytidine, cCyd, was investigated in human colon carcinoma cell line HT-29. Cytidine triphosphate is a pyrimidine nucleoside triphosphate. Here we report the biochemical characterization of Trypanosoma brucei CDA as an enzyme within the tetrameric class of the CDA family that efficiently deaminates cytidine, deoxycytidine, and the nucleoside analogue 5-methyl-2′-deoxycytidine. The T7 RNA polymerase synthesis of modified RNA can be very efficiently used for synthesis of modified RNA but the method has constraints in the sequence of the first three nucleotides of the transcript, which must contain a non-modified G in the +1 position. 36051-68-0 Cytidine 5'-triphosphate is a pyrimidine nucleoside triphosphate that is involved in a variety of biochemical reactions. Tetrahedron 2003 , 59 (37) , 7315-7322. Cytidine 5′-triphosphate (disodium salt) For research use only. It is used in the synthesis of RNA by RNA polymerases. 65-47-4 3D model . Catalog No.S6077 CAS No. CTP is a substrate in the synthesis of RNA. Synthesis of 8-vinyladenosine 5′-di- and 5′-triphosphate: evaluation of the diphosphate compound on ribonucleotide reductase. Cytidine triphosphate (CTP) synthase blockade by CPEC leads to inhibition of smooth muscle cell (SMC) proliferation. Generally, these enzymes hydrolyze some nucleoside 3',5'-cyclic phosphate to some nucleoside 5'-phosphate thus controlling the cellular levels of the cyclic second messengers and the rates of their degradation. It is the rate-limiting enzyme for the synthesis of cytosine nucleotides from both the de novo and uridine salvage pathways.. Cytidine deaminase (CDA) is a pyrimidine salvage enzyme that catalyzes cytidine and deoxycytidine hydrolytic deamination to yield uridine and deoxyuridine. 2',2'-Difluorodeoxycytidine (dFdC, Gemcitabine) is a deoxycytidine analogue which, after phosphorylation to the 5'-di- and 5'-triphosphate (dFdCTP), induces inhibition of DNA synthesis and cell death. Thenucleoside triphosphate pools oftwocytidine auxotrophic mutants ofSal-monella typhimurium LT-2 were studied under different conditions of pyrimidine starvation. Cytidine triphosphate is a pyrimidine nucleoside triphosphate. ; Vreken, P.; Kuilenburg, A.B.P. It is used in the synthesis of RNA by RNA polymerases. diphosphate diglyceride from cytidine triphosphate and phosphatidic acid. Endothelial cells (ECs) have a salvage pathway to synthesize CTP using cytidine, which preserves or promotes proliferation of ECs. Cytidine triphosphate is a pyrimidine nucleoside triphosphate. The Km for cytidine triphosphate was 0.16 mmole/liter and the apparent Km for phosphatidic acid was 6.2 mmoles/liter. The nucleoside triphosphate pools of two cytidine auxotrophic mutants of Salmonella typhimurium LT-2 were studied under different conditions of pyrimidine starvation. novel compounds cytidine diphosphate choline and cytidine diphosphate ethanolamine are examples, has been found to play an essential r&e in the biosynthesis of phospholipides. Chemical Synthesis (1) Molecular Biology (2) Stable Isotopes (4) Special Grade. Cytidine: Guanosine: 8-Oxo-2-deoxyguanosine: Wybutosine: Zebularine ★ Inosine triphosphate - nucleosides .. Add an external link to your content for free. CTP is a substrate in the synthesis of RNA. Now, however, CTP may be useful in the development of … 99% (1) 98% (2) 96% (1) 95% (6) 90 - 94% (1) Physical Form. BP (2) Cytiva (2) EDQM (3) Roche (1) USP (1) Purity. Cytidine triphosphate is a pyrimidine nucleoside triphosphate. Cytidine Triphosphate Synthase Activity and mRNA Expression in Normal Human Blood Cells Verschuur, A.C.; Gennip, A.H. Van; Muller, E.J. Triphosphates like CTP and ATP are high-energy molecules; but ATP is involved in many more metabolic reactions than CTP. Cytidine triphosphate synthetase (CTPS) is the rate-limiting enzyme in de novo CTP synthesis and is required for the formation of RNA, DNA, and phospholipids. Cytidine triphosphate Names IUPAC name [(2R,3S,4R,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl(hydroxy-phosphonooxyphosphoryl)hydrogen phosphate. QTY : Add to my cart. Cytidine triphosphate (CTP) synthetase is a key enzyme for the synthesis of cytosine (deoxy)ribonucleotides, catalysing the conversion of uridine triphosphate (UTP) into CTP, and has a high activity in several malignancies. Academic disciplines Business Concepts Crime Culture Economy Education Energy Events Food and drink Geography … In addition, DP-55 has a requirement for uracil (uridine). CTP is a high-energy molecule similar to ATP, but its role as an energy coupler is limited to a much smaller subset of metabolic reactions. LOpezG.-Coviella I. and Wurtman R. J. Enhancement by cytidine of membrane phospholipid synthesis. HU also exerts a synergistic effect with nucleoside analogs used in the treatment of human immunodeficiency virus (HIV)-1. In the formation of phosphatidylcholine (PC), CTP reacts with phosphocholine, via CTP:phosphocholine cytidylyltransferases, to produce CDP-choline and diphosphate. CDP indicates cytidine diphosphate; CMP, cytidine monophosphate; and UTP, uridine triphosphate. Cytidine triphosphate synthetase (CTPS) catalyzes the rate-limiting step in the de novo synthesis of CTP, and both the yeast and human enzymes have been reported to be regulated by protein kinase A or protein kinase C phosphorylation. certified reference material (1) pharmaceutical primary standard (6) pharmaceutical secondary standard (1) Brand. Cancer cells that exhibit increased cell proliferation also exhibit an increased activity of CTP synthetase. The enzymatic activity could not be dissociated from platelet particles and the greatest specific activity was found in the membrane fraction. An inhibitor of cytidine triphosphate synthesis in human colon carcinoma cells. It is a substrate in the biosynthesis of RNA. Bothmutants,DP45andDP-55,aredefectivein cytidinedeaminaseand cytidine triphosphate (CTP) synthase. Van 1999-01-04 00:00:00 Introduction CTP synthase (EC 6.3.4.2) catalyzes the formation of CTP from uridine triphosphate (UTP) with the concomitant deamination of glutamine to glutamate. Size Price Stock Quantity ; 25mg: EUR 95 In stock: Bulk Size: Bulk Discount: Bulk Inquiry. Search: Add your article Home Carbohydrates Glycosides Glycosides by aglycone type Nucleosides Inosine triphosphate. Other names CTP; Cytidine-5'-triphosphate; Cytidine 5'-(tetrahydrogen triphosphate) Identifiers CAS Number. Reaction mechanism. It is a precursor of cytidine triphosphate (CTP) needed in the phosphatidylcholine (PC) and phosphatidylethanolamine (PE) biosynthetic pathways. Convenient Syntheses of Cytidine 5'-Triphosphate, Guanosine 5'-Triphosphate, and Uridine S'-Triphosphate and Their Use in the Preparation of UDP-glucose, UDP-glucuronic Acid, and GDP-mannose Ethan S. Simon,l Sven Grabowski,2 and George M. Whitesides* Department of Chemistry, Haruard Uniuersity, Cambridge, Massachusetts 02138 Receiued September 20, 1989 This paper compares … Cytidine 5'-triphosphate (CTP) is a pyrimidine nucleoside triphosphate that is involved in a variety of biochemical reactions. Upon exposure of cells to 10(-6)M cCyd, cell viability was reduced to 20% of control, whereas cytocidal activity was not present after 2 hr … In the formation of phosphatidylcholine (PC), CTP reacts with phosphocholine, via CTP:phosphocholine cytidylyltransferases, to produce CDP-choline and diphosphate. Convenient Syntheses of Cytidine 5'-Triphosphate (CTP), Guanosine 5'-Triphosphate (GTP), and Uridine 5'-Triphosphate (UTP) and Their Use in the Preparation of UDP-Glucose, UDP-Glucuronic Acid, and GDP-Mannose Interactive image; Interactive … Cytidine 5'-triphosphate (CTP) is a pyrimidine nucleoside triphosphate that is involved in a variety of biochemical reactions. And UTP, uridine triphosphate CTP ) needed in the body uridine ) CTP acts as inhibitor. But ATP is involved in many more metabolic reactions than CTP the two molecules is the base used, in. ) Supelco ( 1 ) Molecular Biology ( 2 ) Cytiva ( 2 ) (. Km for phosphatidic acid was 6.2 mmoles/liter has a requirement for uracil ( uridine ) hu also a... Activity of CTP synthetase triphosphate ( CTP ) is a pyrimidine nucleoside triphosphate that is involved in many metabolic! Ctp and ATP are high-energy molecules ; but ATP is involved in a variety of biochemical reactions conditions of starvation! 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